diff --git a/ProcessFASTQ.ipynb b/ProcessFASTQ.ipynb index ccdf43a1c332a59b97bd093e8758124455741d96..00480dfdc2bbd81f7ace527108509c519ce0af3e 100644 --- a/ProcessFASTQ.ipynb +++ b/ProcessFASTQ.ipynb @@ -128,6 +128,9 @@ "# This lib is needed to parse fastq easily\n", "from Bio import SeqIO\n", "\n", + "# Prettify arrays\n", + "from pprint import pprint\n", + "\n", "# This lib is used to avoid using a bash magic cell to copy a file\n", "from shutil import copyfile\n", "\n", @@ -683,7 +686,7 @@ }, { "cell_type": "code", - "execution_count": 259, + "execution_count": 262, "metadata": { "collapsed": false }, @@ -698,896 +701,23 @@ "\n", "@PG\tID:Bowtie\tVN:1.1.2\tCL:\"bowtie --wrapper basic-0 -S -v 3 -p 8 --time --best ref/2-Indexes/Yeast-Noncoding/Yeast-Noncoding 3-Filtered/testing.fastq 4-Bowtied/testing.sam\"\n", "\n", - "SN279:493:C84L3ACXX:4:2309:1272:2215\n", - "SN279:493:C84L3ACXX:4:2309:1272:2215:\t4\n", - "SN279:493:C84L3ACXX:4:2309:1275:2239\n", - "SN279:493:C84L3ACXX:4:2309:1309:2236:\t4\n", - "SN279:493:C84L3ACXX:4:2309:2182:2231\n", - "SN279:493:C84L3ACXX:4:2309:1275:2239:\t4\n", - "SN279:493:C84L3ACXX:4:2309:1886:2219\n", - "SN279:493:C84L3ACXX:4:2309:2387:2233:\t4\n", - "SN279:493:C84L3ACXX:4:2309:2846:2219\n", - "SN279:493:C84L3ACXX:4:2309:2846:2219:\t4\n", - "SN279:493:C84L3ACXX:4:2309:3323:2241\n", - "SN279:493:C84L3ACXX:4:2309:3284:2235:\t4\n", - "SN279:493:C84L3ACXX:4:2309:3794:2223\n", - "SN279:493:C84L3ACXX:4:2309:3264:2245:\t4\n", - "SN279:493:C84L3ACXX:4:2309:3694:2243\n", - "SN279:493:C84L3ACXX:4:2309:3794:2223:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4765:2225\n", - "SN279:493:C84L3ACXX:4:2309:4716:2219:\t4\n", - "SN279:493:C84L3ACXX:4:2309:5144:2245\n", - "SN279:493:C84L3ACXX:4:2309:4672:2219:\t4\n", - "SN279:493:C84L3ACXX:4:2309:5407:2223\n", - "SN279:493:C84L3ACXX:4:2309:4765:2225:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4867:2237\n", - "SN279:493:C84L3ACXX:4:2309:5189:2228:\t4\n", - "SN279:493:C84L3ACXX:4:2309:5511:2220\n", - "SN279:493:C84L3ACXX:4:2309:5407:2223:\t4\n", - 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"SN279:493:C84L3ACXX:4:2309:4273:2579\n", - "SN279:493:C84L3ACXX:4:2309:4276:2652:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4443:2564\n", - "SN279:493:C84L3ACXX:4:2309:4404:2715:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4740:2620\n", - "SN279:493:C84L3ACXX:4:2309:4614:2608:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4709:2667\n", - "SN279:493:C84L3ACXX:4:2309:4740:2620:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4404:2715\n", - "SN279:493:C84L3ACXX:4:2309:4555:2703:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4717:2728\n", - "SN279:493:C84L3ACXX:4:2309:4717:2728:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4555:2703\n", - "SN279:493:C84L3ACXX:4:2309:4521:2734:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4521:2734\n", - "SN279:493:C84L3ACXX:4:2309:4818:2514:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4898:2566\n", - "SN279:493:C84L3ACXX:4:2309:4784:2602:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4831:2616\n", - "SN279:493:C84L3ACXX:4:2309:5110:2675:\t4\n", - "SN279:493:C84L3ACXX:4:2309:4784:2602\n", - "SN279:493:C84L3ACXX:4:2309:5205:2692:\t4\n", - "SN279:493:C84L3ACXX:4:2309:5205:2692\n", - "SN279:493:C84L3ACXX:4:2309:5033:2725:\t4\n", - "SN279:493:C84L3ACXX:4:2309:5237:2750\n", - "SN279:493:C84L3ACXX:4:2309:5237:2750:\t4\n", - "SN279:493:C84L3ACXX:4:2309:5033:2725\n", - "SN279:493:C84L3ACXX:4:2309:5270:2523:\t4\n" + "ID: SN279:493:C84L3ACXX:4:2309:1272:2215\n", + "Name: SN279:493:C84L3ACXX:4:2309:1272:2215\n", + "Description: SN279:493:C84L3ACXX:4:2309:1272:2215 1:N:0:CAGATC\n", + "Number of features: 0\n", + "Per letter annotation for: phred_quality\n", + "Seq('NGAGGACCGAACCGACTTACGTT', SingleLetterAlphabet())\n" + ] + }, + { + "ename": "NameError", + "evalue": "name 'pprint' is not defined", + "output_type": "error", + "traceback": [ + "\u001b[1;31m---------------------------------------------------------------------------\u001b[0m", + "\u001b[1;31mNameError\u001b[0m Traceback (most recent call last)", + "\u001b[1;32m<ipython-input-262-737603416198>\u001b[0m in \u001b[0;36m<module>\u001b[1;34m()\u001b[0m\n\u001b[0;32m 22\u001b[0m \u001b[0mprint\u001b[0m\u001b[1;33m(\u001b[0m\u001b[0mrecord\u001b[0m\u001b[1;33m)\u001b[0m\u001b[1;33m\u001b[0m\u001b[0m\n\u001b[0;32m 23\u001b[0m \u001b[0marr\u001b[0m \u001b[1;33m=\u001b[0m \u001b[0mmatches\u001b[0m\u001b[1;33m.\u001b[0m\u001b[0mreadline\u001b[0m\u001b[1;33m(\u001b[0m\u001b[1;33m)\u001b[0m\u001b[1;33m.\u001b[0m\u001b[0msplit\u001b[0m\u001b[1;33m(\u001b[0m\u001b[1;34m'\\t'\u001b[0m\u001b[1;33m)\u001b[0m\u001b[1;33m\u001b[0m\u001b[0m\n\u001b[1;32m---> 24\u001b[1;33m \u001b[0mpprint\u001b[0m\u001b[1;33m(\u001b[0m\u001b[0marr\u001b[0m\u001b[1;33m)\u001b[0m\u001b[1;33m\u001b[0m\u001b[0m\n\u001b[0m\u001b[0;32m 25\u001b[0m \u001b[1;32mbreak\u001b[0m\u001b[1;33m\u001b[0m\u001b[0m\n\u001b[0;32m 26\u001b[0m \u001b[0mseq_id\u001b[0m \u001b[1;33m=\u001b[0m \u001b[0marr\u001b[0m\u001b[1;33m[\u001b[0m\u001b[1;36m0\u001b[0m\u001b[1;33m]\u001b[0m\u001b[1;33m\u001b[0m\u001b[0m\n", + "\u001b[1;31mNameError\u001b[0m: name 'pprint' is not defined" ] } ], @@ -1615,7 +745,10 @@ " seqiter = []\n", " \n", " for record in filt_iter:\n", + " print(record)\n", " arr = matches.readline().split('\\t')\n", + " pprint(arr)\n", + " break\n", " seq_id = arr[0]\n", " flag = arr[1]\n", " if(flag=='4'):\n",